MaGIC Events

Register to join an upcoming event hosted by the Molecular and Genomics Informatics Core! These will include our Technology Seminar Series, any training events, or other fun seminars that may come to be.

Technology Seminar Series

S2 Genomics: Automated solid tissue dissociation for single cell and nuclei ‘omics analyses

June 7, 2023 1:00 PM EST

Single-cell analyses can reveal new levels of complexity of biological systems. The ability to measure the heterogeneity within a system at a single-cell resolution has led to the discovery of new cell types and expanded our understanding of the diverse regulatory pathways and states of individual cells. However, when starting from solid tissues, manual methods to dissociate them reproducibly into high-quality single-cell or nuclei suspensions may be challenging, as tissue-specific protocols may need to be established, be laborious, and with variable yields and viability. Here, we demonstrate the benefits of employing automated solid tissue dissociation into single-cell and -nuclei suspensions from a variety of tissue types. They include enhances in efficiency, reproducibility, and yield, while minimizing manual processing time and potential biases. Through case studies, we discuss the benefits of cell or nuclei isolation depending on application needs, as well as how automated isolation methods may improve sequencing results over manual methods.

Technology Seminar Series

Echo TBD

June 21, 2023 1:00 PM EST